原创 Expressionandbiologicalsignificanceofc-FLIP
2016年06月15日 【健康号】 杜锡林     阅读 6089

       c-FLIP can be considered as a tumor-progression factor in regard to its anti-apoptotic functions. In the present study, we intended to investigate the expression of c-FLIP in human HCC tissues, and its relation with drug-induced cell apoptosis through the specific inhibition of c-FLIP expression by siRNA in 7721 cells.

       Methods: c-FLIP expression was quantified immunohistochemically in HCC tissues(eighty-six cases), and corresponding noncancerous tissues (fifty-seven cases). Patients with HCC were followed up for cancer recurrence.

       Then, the c-FLIP gene was silenced with specific siRNA in 7721 HCC cells. c-FLIP expression was detected by RT-PCR, Western Blot and immunocytochemical staining.

       The cellular viability and cell apoptosis were assayed in vitro with cells treated with doxorubicin. 

       Results: Positive immunostaining was detected for c-FLIP in 83.72% (72/86) human HCC tissues, 14.81% (4/27) hepatic cirrhosis, 11.11% (2/18) hepatic hemangioma tissues, and absent in normal hepatic tissues.

       The overexpression(more than 50%) of c-FLIP in HCC adversely affected the recurrence-free survival. Through c-FLIP gene silencing with siRNA, the expressions of c-FLIP mRNA and protein were remarkably down-regulated in 7721 HCC cells.

       And doxorubicin showed apparent inhibition on cell proliferations, and induced more apoptosis.

       Conclusions: These results indicate that c-FLIP is frequently expressed in human HCCs, and its overexpression implied a lesser probability of recurrence-free survival.

       The specific silencing of c-FLIP gene can apparently up-regulate drug-induced HCC cell apoptosis, and may have therapeutic potential for the treatment of human HCC.

Author: Xilin Du, Guoqiang Bao, Xianli He, Huadong Zhao, Fang Yu, Qing Qiao, Jianguo Lu and Qingjiu Ma

Credits/Source: Journal of Experimental &Clinical Cancer Research 2009, 28:24 book casino

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杜锡林
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唐都医院
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