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谢洋1,刘银华2,周涛1,陈小强1,何仿1
(1.马鞍山市人民医院骨科,安徽 马鞍山 243000;2.皖南医学院弋矶山医院病理科,安徽 芜湖 241000)
[摘要] 目的 观察人体退变椎间盘组织中血小板源性生长因子-A(PDGF-A)、血小板源性生长因子受体-α(PDGFR-α)的表达,探讨其生物学意义。方法 免疫组化(S-P)法分别检测PDGF-A、PDGFR-α、增殖细胞核抗原(PCNA)在正常对照组(A组,n=17)、退变椎间盘组(B组,n=51)中的表达;TUNEL法检测两组椎间盘细胞凋亡情况。结果 PDGF-A、PDGFR-α在B组中表达低于A组,两组之间差异有统计学意义 (P<0.05); 细胞增殖指数(PI) 在B组数值低于A组,细胞凋亡指数(AI)在B组数值高于A组,两组之间差异有统计学意义(P<0.05);PDGF-A、PDGFR-α的表达与PI正相关、与AI负相关(P<0.05);PI、AI值在PDGF-A、PDGFR-α阴性和阳性组中差异有统计学意义(P<0.05)。结论 PDGF-A、PDGFR-α的异常表达与人体椎间盘退变密切相关;PDGF-A、PDGFR-α促进细胞增殖,抑制细胞凋亡,在人体椎间盘退变过程中起了重要的作用,异常表达可能导致椎间盘退变的发生。马鞍山市人民医院骨科谢洋
[关键词] 血小板源性生长因子-A;血小板源性生长因子受体-α;椎间盘退变
Expression and biological significance of PDGF-A and PDGFR-αin human degenerated intervertebral discs
XIE Yang1,Liu Yin-hua2,Zhou Tao1,Chen Xiao-qiang1,He Fang1
(1、Department of Orthopaedics,Maanshan People’s Hospital,Anhui Maanshan 243000,China;2、Department of Pathology,Yijishan Hospital of Wannan Medical College, Anhui Wuhu 241000,China)
[Abstract] Objective To observe the expression of PDGF-A and PDGFR-α in human degenerated intervertebral discs and investigate its biological significance.Methods 68 patients were divided into two groups:17cases of normal intervertebral discs (group A),51 cases of degenerated intervertebral discs(group B).The expression of PDGF-A、PDGFR-α,PCNA and apoptosis were detected by immunohistochemical and TUNEL.Result The expression rate of PDGF-A and PDGFR-αin group B was lower than group A, the difference was significant between them (P<0.05). PI in group B was significant lower than that in group A (P<0.05). AI in group B was significant higher than that in group A (P<0.05). The expression of PDGF-A and PDGFR-αhad positive correlation with PI and negative correlation with AI (P<0.05). There was significant difference in PI and AI between positive expression group and negative expression group (P<0.05). Conclusion Abnormal expression of PDGF-A、PDGFR-αis closely related to degenerative intervertebral discs;PDGF-A and PDGFR-αpromote cell proliferation and inhibit intervertebral discs cell apoptosis, which has an important effect on the degeneration of intervertebral discs.Abnormal expression of PDGF-A、PDGFR-α can induce degeneration of intervertebral discs.
[Key words] platelet-derived growth factor-A;platelet-derived growth factor receptor-α;intervertebral disc degeneration
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